A, Brief Introduction:
First Name: Jian-Guo
Family Name: Tang
Sex: Male
Birth Date: 1963-02-26
Degree: Ph.D.
Phone: 13701015465
/ 10-62987888ext.211
Fax: 10-62980085
Email: [email protected]
Contacting Address:Novozymes China, 14
Xinxi Lu, Shangdi Zone, Haidian District, Beijing 100085, China
Education and Professional Occupations:
1980,9-1985,7 Bachelor of Science in Biochemistry,
Jilin University
1985,9-1990,6 Ph.D., Institute of Biophysics, Academia
Sinica, supervised by Prof. Chen-Lu Tsou
1990,7-2003,3 College of Life Sciences, Peking
University:
Post-doc
(1990-1992)
Associate
Professor (1992-1996)
Professor (1996-
)
Ph.D.
Supervisor (1997-
)
2003,4-present Manager, Application Development Department,
R&D, Novozymes China
Experiences in Abroad:
1994,1-1995,2 Visiting Scholar, University of Cambridge,
UK
1999,1-2000,2 Visiting Scholar, UCLA,
USA
2000,11-2001,2 Visiting
Scholar, University of Melbourne,
Australia
B, Working Experiences:
I have been working in the fields of
protein structure/function relationship, gene engineering, protein engineering,
gene therapy in the last 15 years with about 50 publications (half of them in
western journals) and three patents. The projects include structure/function
and folding pathway of insulin family members, structure/function of trypsin
and leptin, gene engineering of parathyroid hormone, protein engineering of
RGD-containing proteins, gene therapy for diabetes. I have been a group leader
with about 10 people since 1993. I have been also responsible for delivering
lectures to both graduate and undergraduate students. I am now working on new
applications of enzymes in industry.
C, Academic
Awards:
1993 Second
Award for Natural Science Research, Academia Sinica
1994 Beijing Science and Technology Award for Young
People, Beijing Association of Science and Technology
1995 National
Science Fund for Outstanding Young People, Natural Science Foundation of China
1995 National Second Award for Natural Science Research,
Chinese Government
1997 Second Award of Guang Hua Science and Technology -
Ministry level
1998 Award
for Excellence in Teaching for Young People, Peking University
1998 National Science and Technology Award for Young
People, Association of Science and Technology of China
2001 First
Award for Excellent Course on Biochemistry and Molecular Biology in Higher
Education, Beijing Municipal Government
2002 Award
for Outstanding Faculty, Ministry of Education
D, Services in
Academic Society:
2002－ Deputy
Director of the National Laboratory of Protein Engineering and Plant Genetic
Engineering, Peking University
2002－ Vice
President of Beijing Society of Biochemistry and Molecular Biology
1998－ Committee Member, Enzyme Engineering Division,
Chinese Society of Microbiology
1999－ Expert Board for New Drug Evaluation, National
Drug Administration, SDA
2002－ Expert Board for Biochemistry and Molecular
Biology Division, National Natural Science Foundation of China
2000－ Academic Committee Member, Key Laboratory of
Molecular Enzyme Engineering, Jilin University
2000－ Academic Committee Member, Center for Human
Disease Genomics, School of Medicine, Peking University
2000－ Executive Editor, Chinese Journal of
Biochemistry and Molecular Biology
2002－ Editor, Acta Biochimica et Biophysica Sinica
E, Publications:
[1] Tang,J.G.,
Wang,C.C. & Tsou, C.L., (1988) Formation of native insulin
from scrambled molecule by protein disulfide
isomerase., Biochemical Journal, 255, 451-455
[2] Tang,J.G.
& Tsou,C L., (1990) The insulin A and B chains contain structural
information for the formation of the native molecule. Studies with protein
disulfide isomerase., Biochemical Journal, 268, 429-435
[3] 唐建国,
(1991) 蛋白质二硫键异构酶----一种可能参与蛋白质生物合成的酶, 生物化学与生物物理进展, 18, 173-176 (Protein
disulfide isomerase—an enzyme may be
involved in protein biosynthesis, Progress in Biochemistry and Biophysics)
[4] 唐建国,
(1991) 三碘甲腺原氨酸不能与蛋白质二硫键异构酶特异结合, 生物化学杂志, 7, 638-640 (3T-thyroxine does not bind
specifically to protein disulfide isomerase, Chinese Journal of Biochemistry)
[5] 唐建国,
薛英姿, 范贤彬, 付易欣, (1993) 249个氨基酸的人胰岛素原融合蛋白的表达, 生物工程学报, 9, 101-106 (Gene expression
of a human proinsulin fusion protein with 249 amino acids, Chinese Journal of
Biotechnology)
[6] 唐建国,(1993)
用胰蛋白酶转肽的方法使人胰岛素原转成人胰岛素, 生物工程学报, 9, 355-360 (Transpeptidation of human
proinsulin to insulin by trypsin, Chinese Journal of Biotechnology)
[7] Tang,J.G.
& Hu,M.H., (1993) Production of human proinsulin in E.coli in a non-fusion
form., Biotechnology Letters, 15, 661-666
[8] Dai,Y. & Tang,J.G., (1994) Intra-A chain disulfide bond (A6-11) of insulin
is essential for displaying its activity., Biochemistry and Molecular Biology
International, 33, 1049-1053
[9] 唐建国,
张洪涛, 孙怀宇, 戴勇, 胡美浩, (1994) 胰岛素B链N端的修饰及其生物学相关性, 生物化学杂志, 10, 665-669 (Insight into
the importance of insulin B-chain N-terminal by modification, Chinese Journal
of Biochemistry)
[10] 韦革, 张北林, 唐建国,
胡美浩, (1994) 6个氨基酸小C肽人胰岛素原类似物基因的构建及表达, 北京大学学报, 30, 722-727 (Construction and
expression of mini-proinsulin with 6 amino acids as connecting peptide, Acta
Scientiarum Naturalium Universitatis Pekinnensis)
[11] Wei,G., Hu,M.H. & Tang,J.G., (1995) Double-C-peptide human proinsulin. Biochemistry
and Molecular Biology International, 35, 37-46
[12] 李雄彪, 谢斌, 陈来同, 唐建国, 胡美浩, (1995) 人胰岛素原类似物(B-Arg-Arg-A)基因的构建和表达, 北京大学学报, 31, 84-91
(Construction and expression of B-Arg-Arg-A proinsulin, Acta Scientiarum
Naturalium Universitatis Pekinnensis)
[13] Sun,H.Y., Tang,J.G.
& Hu,M.H., (1995) Preparation of Tyr-C-peptide from genetically altered
human insulin precursor., Applied Biochemistry and Biotechnology, 55, 167-174
[14] Chen,J.Q., Zhang,H.T., Hu,M.H. & Tang,J.G., (1995) Production of human
insulin in E. coli system with met-lys-human proinsulin as the expressed
precursor., Applied Biochemistry and Biotechnology, 55, 5-15
[15] Wei,G. & Tang,J.G., (1995) Formation of inclusion bodies may be the key
factor for the stability of the expressed product in E.coli, Biochemistry and
Molecular Biology International, 37, 895-901
[16] 陈来同, 唐建国,
胡美浩, (1995) 重组人胰岛素突变体(GluA4Leu)的分离纯化及性质研究, 生物化学与生物物理进展, 22, 40-43 (Purification
and activity studies of GluA4Leu insulin, Progress in Biochemistry and
Biophysics)
[17] Dai,Y. & Tang,J.G., (1996) Characteristic,
activity and conformational studies of [A6-Ser, A11-Ser]-insulin., Biochimica
et Biophysica Acta, 1296, 63-68
[18] 王军，唐建国，张庭芳，张龙翔，(1996) 胰蛋白酶二硫键重要性的研究，生物化学杂志，12,
583-587 (Role of disulfide bond in trypsin, Chinese Journal of Biochemistry)
[19] 张东裔，唐建国，张龙翔，(1996) 胰蛋白酶活性的定量测定方法，生物化学与生物物理进展，23,
551-553 (A novel method for the activity determination of trypsin, Progress in
Biochemistry and Biophysics)
[20] Li, X.F., Tang, J.G. & Zhang, L.X., Autolytic
site mutant R105C of rat trypsin. (1997) Biochemistry and Molecular Biology
International, 42, 991-996
[21] 刘国安，唐建国，(1997) 人甲状旁腺激素的基因克隆及在大肠杆菌中的表达，生物工程学报，13，264-268
Human parathyroid hormone gene cloning and expression in E. coli, Chinese
Journal of Biotechnology)
[22] Du, X. & Tang, J.G., (1998) Effects of deleting
A19 tyrosine from insulin. Biochemistry Molecular Biology International, 44,
507-513
[23] 唐建国，茹炳根，徐长法，胡美浩，朱圣庚，(1998) 蛋白质工程的研究，北京大学学报，34，342-349
(Insight into protein engineering, Acta Scientiarum Naturalium Universitatis
Pekinnensis)
[24] Du, X. & Tang, J.G., (1998) Hydroxyl group of
insulin A19 tyrosine is essential for receptor binding: studies on (A19-Phe)
insulin. Biochemistry Molecular Biology International, 45, 255-260
[25] Nie, X. & Tang, J.G., (1998) RGD-containing
trypsin with both platelet aggregation inhibitory activity and proteolytic
activity. Biochemistry Molecular Biology International, 45, 1149-1154
[26] 刘国安，唐建国，(1998) 人甲状旁腺激素的研究，生物技术通讯, 9,
145-148 (Progress on the study of human parathyroid hormone, Biotechnology
Communications)
[27] 齐可民，丁宗一，樊征鸿，周红，江载芳，唐建国，(1998) 中国人肥胖抑素cDNA的克隆和在大肠杆菌中的初步表达，中华儿科杂志，36,
40-43 (Leptin gene cloning from Chinese individual and its expression in E.
coli, Chinese Journal of Pediatr.)
[28] Li, X.F., Nie,
X, & Tang, J.G., (1998)
Anti-autolysis of trypsin by modification of autolytic site Arg117. Biochem.
Biophys. Res. Commun., 250, 235-239
[29] Howard, M.J.,
Fuller, C., Broadhurst, R.W., Perham, R.N., Tang, J.G., Quinn, J., Diamond, A.G., & Yeaman, S.J. (1998)
Three-dimensional structure of the major autoantigen in primary biliary
cirrhosis. Gastroenterology, 115, 139-146
[30] 杜欣，唐建国，(1998) 胰岛素受体介导的信号传递，生物化学与生物物理进展，25(5)，43-47
(Signal transduction via insulin receptor, Progress in Biochemistry and
Biophysics)
[31] Yang, Z.H., Du,
X., & Tang, J.G., (1999)
Separation and characterization of trypsin and carboxypeptidase B digested
products of Met-Lys-human proinsulin. Applied Biochemistry and Biotechnology,
76, 107-114
[32] Yuan, Y., Wang,
Z.H., & Tang, J.G., (1999)
Intra-A chain disulfide bond forms first during insulin precursor folding.
Biochemical Journal, 343, 139-144
[33] 陈雅蕙，杜雅励，唐建国，〔1999〕Met-Lys-双C肽人胰岛素原基因的构建表达及分离纯化，中国生物化学与分子生物学报，15，558－562
(Construction and purification of Met-Lys-double-C-peptide proinsulin, Chinese
Journal of Biochemistry and Molecular Biology)
[34] Jing, J., & Tang, J.G., (2000) Platelet aggregation
inhibitory activity of mutant proinsulin with C-peptide replaced by CRGDSC
sequence. Biotechnology Letters, 22, 47-52
[35] 王利民，唐建国，〔2000〕从琼脂糖电泳凝胶中回收DNA的一种简便方法，中国医学遗传学杂志，17，51－53
(A simple method for the recovery of DNA fragment from the gel, Chinese Journal
of Medical Genetics)
[36] 陈新浦，尹东，唐建国，（2000）（A6-Ala, A11-Ala）-人胰岛素突变体的构建及其生物活性，中国生物化学与分子生物学报，16，621－615
(Studies of A6,A11Ala-insulin, Chinese Journal of Biochemistry and Molecular
Biology)
[37] 许婉宁，丁中一，杜丽蓉，唐建国，〔2000〕重组人类肥胖抑素在大肠杆菌中的大规模纯化，中华儿科杂志,
38，365－368 (Expression and purification of human leptin from E. coli, Chinese
Journal of Pediatr.)
[38] Yang,
Z.H., Jing, J., & Tang, J.G.,
(2001) Inhibition of platelet aggregation of a mutant proinsulin molecule
engineered by introduction of a native Arg-Gly-Asp sequence. Applied
Biochemistry and Biotechnology, 90, 1-10
[39] Yin,
D., & Tang, J.G., (2001) Gene
therapy for streptozotocin-induced diabetic mice by electroporational transfer
of naked human insulin precursor DNA into skeletal muscle in vivo. FEBS
Letters, 495, 16-20
[40] Tang, J. G., & Koeffler, H.P., (2001) Structural and functional
studies of CCAAT/Enhancer-binding protein epsilon. J Biol Chem.,276, 17739-46
[41] 陈历明，唐建国，（2002）分子内分子伴侣－Pro肽在蛋白质折叠中的作用，生命科学，14，35－39 (Intra-molecular
chaperon—role of pro peptide in protein folding, Chinese Bulletin of Life
Sciences)
[42] 陈历明，杨兴文，唐建国，（2002）人松弛素原样蛋白在大肠杆菌中的表达、分离纯化及鉴定，生物化学与生物物理学报，34,
595-600 (Expression, purification and characterization of human
prorelaxin-like-protein H2 in E. coli, Acta Biochimica et Biophysica Sinica)
[43] Chen, L.M., Yang, X.W.,
& Tang, J.G. (2002) Acidic
residues on the N-terminus of proinsulin C-peptide are important for the
folding of insulin precursor. J Biochem., 131, 855-59
[44] Yang,
J.C., Liu, J., Yang, X.W., & Tang,
J.G. (2002)Gene therapy for diabetic rats by electroporational transfer of
naked plasmid with human preproinsulin gene into skeletal muscle. Biotechnology
Letters, 24, 851-855
[45] 杨兴文，刘俭，唐建国，（2002）抗凝活性肽RGD226基因构建、表达、产物纯化及活性分析，中国生物化学与分子生物学报,
18, 583-587 (Gene construction, expression, purification and activity analysis
of anti-platelet aggregation peptide RGD226, Chinese
Journal of Biochemistry and Molecular Biology)
[46] Liu, Y., & Tang, J.G. (2003) Influence of A7-B7 disulfide bond deletion on the
refolding and structure of proinsulin. Acta Biochimica et Biophysica Sinica,
35(2), 122-126
[47] Liu, J., Yang, X.W., Chen, W.Q., & Tang, J.G. (2003) Effect of disulfide
bond formation on the conformation and anticoagulant activity of an Arg-Gly-Asp
motif displayed on a mutant insulin protein framework. Letters in Peptide
Science, in press
[48] Tang, J.G., Wang, Z.H., Tregear, G.W., & Wade, J.D. (2003)
Human gene 2 relaxin chain combination and folding. Biochemistry, 42(9),
2731-2739
[49] Wang,
Z.H., Liu, Y., Yang, X.W., & Tang,
J.G. (2003) Probing the role of disulfide bonds in insulin folding and
activity. In preparation
[50] Wang, X.D., Liu, J., Yang, J.C., Chen, W.Q.,
& Tang J.G. (2003) Mice body
weight gain is prevented after naked human leptin cDNA transfer into skeletal
muscle by electroporation. In preparation
[51] Yang, J.C. & Tang J.G. (2003) Aromatic side chain of Tyr61 is
important for structure and function of leptin. In preparation
F, Patents:
[1]
唐建国, 胡美浩, 胰岛素前体基因在大肠杆菌中的直接表达及后加工方法, 中国专利, 专利号:
93103997.5 (Direct expression of insulin precursor gene in E. coli and simple procedure
for preparation of human insulin)
[2] 唐建国，井健，杨志宏，以无活性胰岛素为骨架的含RGD序列的抗栓新药，中国专利，申请号：99109419.0
(Anticoagulant RGD motif displayed on an inactive insulin protein framework,
patent in pending)
[3] 唐建国，王宪，尹东，一种用于直接肌肉注射治疗糖尿病的人胰岛素前体基因和含有该基因的质粒，中国专利，申请号：00129717.1
(A plasmid with insulin precursor gene for treatment of diabetes by electroporational
transfer into skeletal muscle, patent in
pending)
G, Book：
《生物化学》第三版，（2002）王镜岩
朱圣庚 徐长法 主编， 高等教育出版社，本人完成4章写作。(A text book on Biochemistry used national wide, third
edition, I contributed 4 chapters. Published by Higher Education Press)